Protective humoral responses to severe acute respiratory syndrome-associated coronavirus: implications for the design of an effective protein-based vaccine.
Identifieur interne : 005183 ( Main/Exploration ); précédent : 005182; suivant : 005184Protective humoral responses to severe acute respiratory syndrome-associated coronavirus: implications for the design of an effective protein-based vaccine.
Auteurs : Hai Pang [République populaire de Chine] ; Yinggang Liu [République populaire de Chine] ; Xueqing Han [République populaire de Chine] ; Yanhui Xu [République populaire de Chine] ; Fuguo Jiang [République populaire de Chine] ; Donglai Wu [République populaire de Chine] ; Xiangang Kong [République populaire de Chine] ; Mark Bartlam [République populaire de Chine] ; Zihe Rao [République populaire de Chine]Source :
- The Journal of general virology [ 0022-1317 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- immunologie : Protéines virales, Vaccins antiviraux, Virus du SRAS.
- sang : Anticorps antiviraux.
- Animaux, Cellules Vero, Lapins, Mâle, Syndrome respiratoire aigu sévère, Tests de neutralisation.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antibodies, Viral.
- immunology : SARS Virus, Viral Proteins, Viral Vaccines.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Male, Neutralization Tests, Rabbits, Vero Cells.
Abstract
Some of the structural proteins of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) carry major epitopes involved in virus neutralization and are essential for the induction of protective humoral responses and the development of an effective vaccine. Rabbit antisera were prepared using full-length N and M proteins and eight expressed fragments covering the S protein. Antisera to S and M proteins were found to have different neutralizing titres towards SARS-CoV infection in vivo, ranging from 1:35 to 1:128. Antiserum to the N protein did not contain neutralizing antibodies. Epitopes inducing protective humoral responses to virus infection were located mainly in the M protein and a region spanning residues 13-877 of the S protein. The neutralizing ability of antisera directed against the expressed structural proteins was greater than that of convalescent patient antisera, confirming that, as immunogens, the former induce strong, SARS-CoV-specific neutralizing antibody responses. The in vitro neutralization assay has important implications for the design of an effective, protein-based vaccine preventing SARS-CoV infection.
DOI: 10.1099/vir.0.80111-0
PubMed: 15448374
Affiliations:
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of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Yinggang" sort="Liu, Yinggang" uniqKey="Liu Y" first="Yinggang" last="Liu">Yinggang Liu</name><affiliation wicri:level="1"><nlm:affiliation>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Han, Xueqing" sort="Han, Xueqing" uniqKey="Han X" first="Xueqing" 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wicri:level="3"><nlm:affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name><affiliation wicri:level="3"><nlm:affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Chlorocebus aethiops</term><term>Male</term><term>Neutralization Tests</term><term>Rabbits</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Vero Cells</term><term>Viral Proteins (immunology)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Cellules Vero</term><term>Lapins</term><term>Mâle</term><term>Protéines virales (immunologie)</term><term>Syndrome respiratoire aigu sévère ()</term><term>Tests de neutralisation</term><term>Vaccins antiviraux (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines virales</term><term>Vaccins antiviraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Viral Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Male</term><term>Neutralization Tests</term><term>Rabbits</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Lapins</term><term>Mâle</term><term>Syndrome respiratoire aigu sévère</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Some of the structural proteins of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) carry major epitopes involved in virus neutralization and are essential for the induction of protective humoral responses and the development of an effective vaccine. Rabbit antisera were prepared using full-length N and M proteins and eight expressed fragments covering the S protein. Antisera to S and M proteins were found to have different neutralizing titres towards SARS-CoV infection in vivo, ranging from 1:35 to 1:128. Antiserum to the N protein did not contain neutralizing antibodies. Epitopes inducing protective humoral responses to virus infection were located mainly in the M protein and a region spanning residues 13-877 of the S protein. The neutralizing ability of antisera directed against the expressed structural proteins was greater than that of convalescent patient antisera, confirming that, as immunogens, the former induce strong, SARS-CoV-specific neutralizing antibody responses. The in vitro neutralization assay has important implications for the design of an effective, protein-based vaccine preventing SARS-CoV infection.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Pang, Hai" sort="Pang, Hai" uniqKey="Pang H" first="Hai" last="Pang">Hai Pang</name></noRegion><name sortKey="Bartlam, Mark" sort="Bartlam, Mark" uniqKey="Bartlam M" first="Mark" last="Bartlam">Mark Bartlam</name><name sortKey="Han, Xueqing" sort="Han, Xueqing" uniqKey="Han X" first="Xueqing" last="Han">Xueqing Han</name><name sortKey="Jiang, Fuguo" sort="Jiang, Fuguo" uniqKey="Jiang F" first="Fuguo" last="Jiang">Fuguo Jiang</name><name sortKey="Kong, Xiangang" sort="Kong, Xiangang" uniqKey="Kong X" first="Xiangang" last="Kong">Xiangang Kong</name><name sortKey="Liu, Yinggang" sort="Liu, Yinggang" uniqKey="Liu Y" first="Yinggang" last="Liu">Yinggang Liu</name><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name><name sortKey="Wu, Donglai" sort="Wu, Donglai" uniqKey="Wu D" first="Donglai" last="Wu">Donglai Wu</name><name sortKey="Xu, Yanhui" sort="Xu, Yanhui" uniqKey="Xu Y" first="Yanhui" last="Xu">Yanhui Xu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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